Journal: Journal of neuroscience methods
Article Title: A manual multiplex immunofluorescence method for investigating neurodegenerative diseases
doi: 10.1016/j.jneumeth.2020.108708
Figure Lengend Snippet: Photomicrographs of the validation experiments for antibody CP13. Overlapping AF647 and AF488 signals represents antigen preservation. Lack of AF546 signal represents successful stripping, which was achieved only for heat and β-mercaptoethanol/SDS strategies (A and D) strategies. The Glycine/SDS, pH 2.0 strategy (B) resulted in a reduced, but noticeable AF546 signal, and denaturing solution strategy (C) resulted in a significant AF546 signal. Scale bar: 10 μm
Article Snippet: Denaturing solution , One part of solution A to two parts of solution B from the Denaturing Solution Kit from Biocare Medical , Manufacturer instructions (part DNS001L).
Techniques: Biomarker Discovery, Preserving, Stripping Membranes